Photosynthetic GFS-3000 and dual-channel PAM-100 measurement system are used in combinationThe measurement of gas exchange, chlorophyll fluorescence and differential absorption can be achieved simultaneously.Synchronous measurementsco2Gas exchangeandP700system, synchronous measurementco2Gas exchange,P700andChlorophyll fluorescencesystem.
System composition
Main functions
ComeSynchronous measurement of P700, chlorophyll fluorescence and gas exchange
ComeSynchronous measurement of dark-light induction curves of P700, chlorophyll fluorescence and gas exchange
ComeSynchronous measurement of photoresponse curves and CO of P700, chlorophyll fluorescence and gas exchange2Response curve
ComeTypical gas exchange measurements such as photosynthesis, transpiration, respiration
ComeTypical chlorophyll fluorescence measurements, such as induction curves, rapid light curves, quenching analysis, dark relaxation, etc.
ComeTypical P700 curve measurements
ComeChlorophyll fluorescence and rapid induction kinetics of P700, etc.
ComeProgramming for complex synchronous or independent measurements
Measurement parameters
PS IIparameter:Fo, Fm, F, Fm’, Fv/Fm, Y(II)=△F/Fm’, Fo’, qP, qL, qN, NPQ, Y(NPQ), Y(NO) and ETR(II), etc.
psiparameter:P700, Pm, Pm’, P700red, Y(I), Y(ND), Y(NA) and ETR(I), etc.
Gas exchange parameters:CO in reference chamber and sample chamber2Absolute value (CO2ABS, co2sam), reference chamber and sample chamber H2O Absolute Value (H2OA logo, H2Osam), flow rate (gas flow), ambient air pressure (Pamb), leaf chamber temperature (Tcuv), blade temperature (Tleaf), ambient temperature (Tamb), ambient PAR (PARamb), leaf indoor blade front PAR (PARtop), leafPAR (PARbot) on the back of the indoor blade, relative humidity of the leaf chamber (rH), transpiration rate (E), water pressure saturation deficiency (VPD), blade pore conductivity (GH2O), net photosynthetic rate (A), intercellular CO2Concentration (Ci), environmental CO2Concentration (Ca), plant moisture utilization efficiency, CO2Response curve, light response curve, etc.
Designed for synchronous measurements between DUAL-PAM-100 and GFS-3000, it consists of a special blade chamber (with temperature and PAR sensor), fan, light guide rod, electronic box and bracket.During synchronous measurements, the light source is provided entirely by the DUAL-PAM-100's measuring head, the gas exchange is detected by the GFS-3000's infrared analyzer, and the P700 and chlorophyll fluorescence is measured by the DUAL-PAM-100's detector.
It should be noted that the 3010-DUAL can be connected to the DUAL-DB measuring head of the DUAL-PAM-100, but cannot be connected to the DUAL-DR measuring head.The optical unit of DUAL-DR is too complex, and connecting 3010-DUAL can easily damage DUAL-DR.
Measurement example
The two pictures below are of ivy (helix) is a material, and the induction curves of P700, chlorophyll fluorescence and gas exchange were measured synchronously using this system.
Ivy (helix)ofP700 (blue)andChlorophyll fluorescence (red)induction curve
Ivy (helix)ofNet photosynthetic rate (red)andStomatal conductivity (blue)induction curve
Synchronous measurementco2Gas exchange and transmembrane proton dynamic potential (pmf), transmembrane proton gradient (ΔpH), transmembrane potential (ΔΨ) and zeaxanthin (Zea)
The P515/535 module is a measurement module designed by WALZ for DUAL-PAM-100. It can be directly connected to the host of DUAL-PAM-100 to measure the difference absorption of 550-510 nm and the signal changes of 535 nm wavelength.The P515/535 module can measure the transmembrane proton dynamic potential (pmf), transmembrane potential (Δψ), transmembrane proton gradient (ΔpH) and zeaxanthin (Zea) changes of photosynthetic organs.In addition, the module also provides a special "P515 Flux" operation mode that allows actinic light to turn on and off in light-dark pulse form (1/1 modulated light/dark), in situ measurement of live samples in steady stateThe flow rate of coupled electrons and protons.
After the GFS-3000 is used in a dedicated gas exchange leaf chamber 3010-DUAL, DUAL-PAM-100 and P515/535 module, the GFS-3000 can be used simultaneously to measure CO.2Gas exchange and transmembrane proton dynamic potential (pmf), transmembrane proton gradient (ΔpH), transmembrane potential (ΔΨ) and zeaxanthin (Zea).Since ΔpH and Zea are closely related to lutein circulation, lutein circulation is an important regulatory mechanism for plant photo protection. Combined with the Calvin circulation reflected by gas exchange indicators, the plant photo protection regulation mechanism can be carried out in a very deep mannerResearch.This synchronous measurement technology has just emerged and no literature has been published yet.
