firesTingO2As a revolutionary technology for oxygen measurement, it is particularly suitable for the measurement of photosynthetic oxygen release and soil microecology research.FireSting O2, the latest compact fiber-optic oxygen measuring instrument developed by German Pyro Science, has extremely high measurement accuracy and can be operated separately through USB connection PC control software.Oxygen release and chlorophyll fluorescence can also be measured simultaneously with MINI-PAM-II. |  | ||||||||
| A variety of optional probes can flexibly meet the needs of various O2 measurements and can be used for O2 concentration measurement in containers, vials and mobile phases.The needle-shaped probe can also measure the distribution of O2 concentration deep into the algae tissue. |  | ||||||||
Application Example: Blu-ray version MINI-PAM-II records Chlorella chlorophyll fluorescence and oxygen release simultaneously | |||||||||
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A:The picture shows Chlorella12One light intensity, each level of light intensity gradient irradiation5 peopleThe fluorescence intensity of modulated chlorophyll (red line) and oxygen concentration (blue line) shown changes.The light intensity of this series of light intensity gradients (par) Approximately exponential growth.From left to right,YThe axes represent light intensity μTouched-2 is-1, fluorescence intensity (relative intensity, no unit), relative oxygen saturation (100%saturation= 273μM),FMandFM’The horizontal is represented by purple dots.FandFThe horizontal is indicated by green dots. B: The relative electron transfer rate (red symbols and lines) is related to the quantum ΦPSII and PAR of the optical system II, which is calculated from Fm and Fm’ in Figure A (see Genty et al. 1989).Oxygen (blue line) is the first derivative of the curve in Figure A, which corresponds to the change in relative oxygen saturation per minute. The light blue symbol represents the measurement data of relative oxygen saturation within 3 minutes under the corresponding light intensity gradient. set up: Chlorella in the suspension sample chamber KS-2500 was measured using MINI-PAM-II/B, and a needle-shaped opti-cal oxygen sensor connected to the oximeter was inserted into the suspension sample chamber KS-2500 from a hole on the side.Use a new adapter to connect the oximeter and MINI-PAM-II/B together, and the data of oxygen and fluorescence are recorded synchronously. significance: This device realizes synchronous recording of chlorophyll fluorescence and oxygen concentration. From the fluorescence data, we can obtain photosynthetic linear electron transfer rate, but the change in oxygen concentration is affected by the dual influence of oxygen release and consumption. Therefore, the electron transfer rate and oxygen are measured simultaneously.The relationship between photosynthesis, photorespiration, and dark breathing can be studied in depth. | |||||||||
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