Main functions

• Fluorescence induction curve can be measured and quenched analysis

• Meterable light response curve and fast light curve (RLC)

• All use LED light sources, new touch screen design

• Added humidity measurement function and added far red light source

• The instrument is more portable and suitable for field measurement

• Can be used in combination with a photosynthesis device (such as GFS-3000) through micro-fiber

• Powered by No. 5 battery, easy to obtain backup battery, suitable for long-term outdoor use

Measurement parameters

Fo, Fm, F, Fm', Fo', Fv/Fm, Y(II) i.e. ΔF/Fm', qP, qL, qN, NPQ, Y(NPQ), Y(NO), rETR, PAR, leaf WenheRelative humidity, etc.

Application areas

Study the mechanism of photosynthesis, the impact of various environmental factors (light, temperature, nutrition, etc.) on the physiological ecology of plants, stress resistance of plants (drought, cold, heat, UV, virus, pollution, etc.), long-term ecological changes of plants, etc..

It has been widely used in the fields of plant physiology, plant ecology, plant pathology, agronomy, forestry, horticulture, aquatic biology, environmental science, etc.

Main technical parameters

• Measured light: Blue LED (470 nm) or Red LED (655 nm), light intensity 0.05 µmol m^-2yes^-1.

• Actinic light: blue light LED (470 nm) or red light LED (655 nm), maximum continuous light intensity 3000 μmol m^-2yes^-1.

• Saturation pulse: Blue LED (470 nm) or Red LED (655 nm), maximum flash intensity 6000 μmol m^-2yes^-1.

• Far-red light: emission peak 740 nm.

• Signal detection: PIN-photodiode with long pass and short pass filters with selective phase lock amplifier.

• Data storage: 8M memory card, which can store 27,000 sets of data.

• Leaf clip: The 2035-B Leaf clip contains MINI-PAM/F fiber placement port and sample clip.The upper and lower parts of the leaf clamp can be clamped after opening it.The upper part of the clip provides a circular measuring area with a diameter of 1 cm.The standard distance between the fiber tip and the measurement area is 8 mm.The optical fiber is at a 60° angle to the measurement plane.The blade temperature sensor is installed below the measuring area.The humidity sensor is installed at a distance of 3cm from the measuring area.The built-in chip saves the calibration data of the sensor.The saturation pulse can be released through the remote trigger button.An additional light sensor input interface is also provided.PAR measurement range 0-7000 µmol m^-2yes^-1, blade temperature measurement range -20 to +60℃, humidity measurement range 0-100% RH.

• Power supply: 6 AA (No. 5 1.2 V/2 Ah) rechargeable batteries, which can provide 1000 saturation pulse flashes at one time.

Purchase Guide

1. Basic article on measuring leaves of higher plants

System composition: host, optical fiber, light adaptive leaf clip, dark adaptive leaf clip, software, etc.

Note: One of the Red Light and Blu-ray (recommended) hosts can be selected

Basic article on leaf measurement of higher plants

2. Basic hanging sample measurement

System composition: host, optical fiber, suspended sample chamber, magnetic stirrer, optical fiber oxygen meter (optional), software, etc.

Note: When measuring algae, it is recommended to choose the Red Light Mode host.

Basic hanging sample measurement model

Initial interface	Basic measurement result display interface	Detailed parameter display interface
Real-time fluorescence display interface	Slow induced kinetic curve interface	Fast light curve display interface

3. Other optional accessories

• External LED light source: 2054-L.

• It can be connected to the 2035-B leaf clip to provide external actinic light, red, green, blue and white, with maximum excitation wavelengths of 630 nm red, 520 nm green, 452 nm blue and white light with a wavelength range of 450nm-680nm.The maximum light intensity for each wavelength is 1500µmol m^-2yes^-1When connected to an external power supply device, the overall maximum light intensity can reach 6000µmol m^-2yes^-1Color combinations are free to choose.

• 90 degree angle fiber adapter: 2030-B90.

• Installed on 2030-B or 2060-B, bringing the fiber to a 90-degree angle to the sample.

• Miniature photo quantum/temperature sensor: 2065-M.

• Measure PAR and temperature, can be used independently after connecting to MINI-PAM, and is mostly used in combination with 2060-B.

• Large sample surface fiber fixing bracket: 2060-A.

• Fix the fiber to the surface of the larger sample (non-blade) and use in conjunction with 2065-M.

• Arabidopsis leaf clip: 2060-B.

• 60-degree angle light adaptation leaf clips, used in conjunction with independent micro-optical quantum/temperature sensor 2060-M, especially suitable for measuring Arabidopsis leaflets.Requires 2060-M configuration.

• Micro-optic fiber: MINI-PAM/F1.

• Diameter 2 mm and length 1.5 m for measuring small samples.Includes an adapter connected to the 2035-B.

Application example

Origin: WALZ, Germany

References

Data source: Photosynthesis Literature Endnote database, updated to September 2016, with more than 6,000 documents

Original data source: Google Scholar

Note: MINI-PAM-II is a newly upgraded product of MINI-PAM. For more documents, please refer to the MINI-PAM Document Catalog.

Bai, X., et al. (2016). "Proteomic analysis brings new insights into the effect of a dark stress on lipid biosystemthesis in Phaeodactylum tricornutum." Scientific Reports 6: 25494. [MINI-PAM-II]

Bai, X., et al. (2015). "Effects of atrazine on photosynthesis and defense response and the underlying mechanisms in Phaeodactylum tricornutum." Environmental Science and Pollution Research 22(22): 17499-17507. [MINI-PAM-II]

Jin, Y., et al. (2016). "Diuron treatment reveals the different roles of two cyclic electron transfer pathways in photosystem II in Arabidopsis thaliana." Pesticide Biochemistry and Physiology. [MINI-PAM-II]

l i, H., ETA. (2015). "effect of hydrogen sulfide on D1 protein in wheat under drought stress." act AP also needs SiO logia E plan to step into M 37(11): 1-9. [mini-Pam-II]

Li, H., et al. (2016). "High temperature effects on D1 protein turnover in three wheat variety with different heat susceptibility." Plant Growth Regulation: 1-9. [MINI-PAM-II]

Rajendran, D. K., et al. (2016). "Visual Analysis for Detection and Quantification of Pseudomonas cichorii Disease Severity in Tomato Plants." The Plant Pathology Journal 32(4): 300-310. [MINI-PAM-II]

Saada, G., et al. (2016). "Taking the heat: distinct vulnerability to thermal stress of central and threatened peripheral lines of a marine macroalga." Diversity and Distributions: n/a-n/a. [MINI-PAM-II]

Song, H., et al. (2016). "Inhibitory effects of tributyl phosphate on algal growth, photosynthesis, and fatty acid synchronization in the marine diagnosis Phaeodactylum tricornutum." Environmental Science and Pollution Research: 1-10. [MINI-PAM-II]

Xu, C. and B. Mou (2016). "Vermicompost Affects Soil Properties and Spinach Growth, Physiology, and Nutritional Value." HortScience 51(7): 847-855. [MINI-PAM-II]

Gu Junfei, et al. (2016). "The effect of mutations in low chlorophyll content in rice on photosynthesis and yield." Acta Crop Sinica. [MINI-PAM-II]

Zhou Zhenxiang, et al. (2016). "The effect of reduced chlorophyll content on photoinhibition and photosynthesis electron transfer in rice leaves." China Agricultural Sciences 49(19): 3709-3720.[MINI-PAM-II]