Main functions
Imaging function: Ft, Fo, Fm, Fv/Fm, F, Fm', Y(II), Y(NO), Y(NPQ), NPQ, qN, qP, qL, ETR, Abs., NIR, RedAt least 17 parameters were used for imaging analysis.The regulation energy dissipation Y (NPQ) is measured to reflect the plant's light protection ability, and the non-regulated energy dissipation Y (NO) is measured to reflect the degree of plant light damage.
Program measurement function: can program the measurement of fluorescence induction curves, fast light curves and dark relaxation, or manually measure; it can automatically analyze the changing trends of all fluorescence parameters during the measurement process.
AOI function: The region of interest (AOI) can be selected arbitrarily before or after measurement. The program will automatically analyze the change trend of the selected AOI data and display the data of the relevant AOI in the report file.Data displayed in all report files can be exported to the EXCEL file.
Imaging heterogeneity analysis function: For imaging any parameter and arbitrary time, two points can be selected on the image. The software automatically performs horizontal heterogeneity analysis on the data between the two points and can be exported to the EXCEL file.
Imaging data range analysis function: For imaging of any parameter and arbitrary time, you can analyze how many pixels are there between any two fluorescence values and how many areas (cm)2).
Mutant strain screening function: According to the imaging results, mutant strains such as photosynthetic, hydrogen/oil production, stress resistance (salt resistance, drought resistance, disease resistance, etc.) can be quickly screened.
Microalgae toxicology research function: The photosynthetic activity of 96 microalgae samples (control and treatment groups) can be measured simultaneously, and the software automatically gives the percentage of photosynthetic inhibition of the treatment group samples relative to the control group.
Absorption coefficient measurement function: Quickly measure the absorbance coefficient of the blade.Absorption coefficient measurement light sources: 16 red light (650 nm) and 16 near-infrared (780 nm) LEDs for measuring PAR absorption coefficients in plant leaves or algae samples.
Measurement parameters
Ft, Fo, Fm, Fv/Fm, F, Fm’, Y(II), Y(NO), Y(NPQ), NPQ, qN, qP, qL, ETR, Abs., NIR, Red, etc.
Application areas
Photosynthesis study: A large number of samples can be imaged simultaneously under exactly the same conditions
Plant pathology: imaging of lesions (including when they are not visible to the naked eye) and spatiotemporal dynamics of lesions diffusion
Plant stress physiology: Early detection of stress damage invisible to naked eyes
Genetic breeding: Large-scale rapid screening of high photosynthetic/drought resistance/thermal resistance/freeze resistance and disease resistance after seedling emergence
Mutant strain screening: Rapid screening of photosynthetic mutant strains, stress-resistant mutant strains, hydrogen-producing microalgae mutant strains, etc.
Microalgae Toxicology: Multiple repeated samples of different toxic concentrations are measured in one go, and the software automatically calculates the inhibition ratio
Molecular Biology: Detection of Green Fluorescent Protein (GFP) Fluorescence of Samples at the Macroscopic Level
Various other extended research
Main technical parameters
MAXI probe (Blue lightversion)
Fluorescence measurement light source:44 blue LEDs, 450 nm, measure light intensity 0.5 μmol m-2yes-1, maximum actinic light intensity 1200 μmol m-2yes-1, saturation pulse intensity 2800 μmol m-2yes-1.
Absorption coefficient measurement light sources: 16 red light (650 nm) and 16 near-infrared (780 nm) LEDs for measuring the PAR absorbance coefficient of the sample.
Imaging area: 10×13 cm.
Light intensity heterogeneity: The measured area light intensity heterogeneity is less than ±7%.
Measurement parameters: Ft, Fo, Fm, Fv/Fm, F, Fm', Y(II), Y(NO), Y(NPQ), NPQ, qN, qP, qL, ETR, Abs., NIR, Red, etc..
MAXI probe (Red lightversion)
Fluorescence measurement light source:44 red LEDs, 620 nm, measure light intensity 0.5 μmol m-2yes-1, maximum actinic light intensity 1200 μmol m-2yes-1, saturation pulse intensity 2800 μmol m-2yes-1.
Absorption coefficient measurement light sources: 16 red light (650 nm) and 16 near-infrared (780 nm) LEDs for measuring the PAR absorbance coefficient of the sample.
Imaging area: 10×13 cm.
Light intensity heterogeneity: The measured area light intensity heterogeneity is less than ±7%.
Measurement parameters: Ft, Fo, Fm, Fv/Fm, F, Fm', Y(II), Y(NO), Y(NPQ), NPQ, qN, qP, qL, ETR, Abs., NIR, Red, etc..
MINI probe (Blue lightMINI probe imaging is convenient for field use)
Fluorescence measurement light source:12 blue LEDs, 460 nm, measure light intensity 0.5 μmol m-2yes-1, maximum actinic light intensity 2000 μmol m-2yes-1, saturation pulse intensity 6000 μmol m-2yes-1.
Absorption coefficient measurement light sources: 10 red light (650 nm) and 10 near-infrared (780 nm) LEDs for measuring the PAR absorbance coefficient of the sample.
Signal detection: 1/3” digital camera CCD, 640×480 pixels, acquisition rate of 30 frames/sec, with selective phase lock amplifier. Communication with computer data via Gigabit Ethernet (GigE).
Imaging area: 24×32 mm, 6x enlargement.
Measurement parameters: Ft, Fo, Fm, Fv/Fm, F, Fm', Y(II), Y(NO), Y(NPQ), NPQ, qN, qP, qL, ETR, Abs., NIR, Red, etc..
MINI probe (Red lightMINI probe imaging is convenient for field use)
Fluorescence measurement light source:12 red LEDs, 620 nm, measure light intensity 0.5 μmol m-2yes-1, maximum actinic light intensity 2000 μmol m-2yes-1, saturation pulse intensity 6000 μmol m-2yes-1.
Absorption coefficient measurement light sources: 10 red light (650 nm) and 10 near-infrared (780 nm) LEDs for measuring the PAR absorbance coefficient of the sample.
Signal detection: 1/3” digital camera CCD, 640×480 pixels, acquisition rate of 30 frames/sec, with selective phase lock amplifier. Communication with computer data via Gigabit Ethernet (GigE).
Imaging area: 24×32 mm, 6x enlargement.
Measurement parameters: Ft, Fo, Fm, Fv/Fm, F, Fm', Y(II), Y(NO), Y(NPQ), NPQ, qN, qP, qL, ETR, Abs., NIR, Red, etc..
Purchase Guide
1. Fluorescence Imaging MAXI Edition
System composition: general-purpose host, LED power supply unit, LED light source, CCD, data line, software, etc.
Note: Selected when measuring higher plants or eukaryotic algaeBlue lightLight source (recommended), please select cyanobacteria measurementRed lightlight source.
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| Fluorescence Imaging MAXI Version | |
2. Fluorescence imaging MINI version
System composition: general-purpose host (not shown in the figure below), LED light source (as shown below), CCD, data cable, software, etc.
Note: Selected when measuring higher plants or eukaryotic algaeBlue lightLight source (recommended), please select cyanobacteria measurementRed lightlight source.
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| Fluorescence Imaging MINI Edition |
3. Fluorescence imaging MICROSCOPY version
System composition: general-purpose host, special microscope, CCD, data cable, software, etc.
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| Fluorescence Imaging MICROSCOPY Edition |
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| Grape leaf Y(NPQ) imaging | Grape Leaf PS/50 Imaging Results | MINI version imaging results |
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| Microscopic version imaging results | Software imaging interface | Slow induction kinetic curve |
Origin: WALZ, Germany
References
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