Integration of cell culture and detection
Product Principle
The core technology of the intelligent cell real-time monitor is cell detection based on the principle of electrical impedance sensors, which can monitor the dynamic biological reaction processes of cells such as growth, proliferation, toxicity, adhesion and morphological changes in real time.It mainly consists of three parts: cell analyzer, real-time cell analyzer control software and 16-well cell detection plate.During the experiment, the cell analyzer was placed on CO2In the incubator, a micro-gold electronic sensor chip is integrated at the bottom of the cell detection plate. When cells grown on the surface of the microelectrode cause changes in the electrode interface impedance, the change in the impedance value directly reflects the biological status of the cells.The computer can control the operation of the entire system online and perform data analysis.Therefore, CM100-α tracks cell morphology and proliferation and differentiation changes in real-time, continuously and quantitatively under the physiological state of cells, providing a unique method for real-time dynamic information acquisition for cell-level analysis and research.
Uses of instruments
CM100-α——Intelligent cell real-time monitor can be widely used in various disciplines such as cell biology, molecular biology, oncology, biochemistry, toxicology, and drug screening, R&D, production and quality control processes.
Intelligent cell real-time monitors can monitor the physiological status and behavior of cells in real time.By measuring the electrical impedance changes of cells, information on cell growth, proliferation, differentiation, death and other information can be obtained, which helps researchers understand the physiological and pathological processes of cells and the effects of drugs on cells.Drug screening and pharmacodynamic efficacy study: Cell growth online detectors can be used for drug screening and pharmacodynamic efficacy study.By observing the electrical impedance changes of cells before and after drug treatment, the effect of drugs on cells can be evaluated, providing strong support for the development of new drugs.
Features
● By detecting the electrical impedance change trends caused by cell growth, division, apoptosis, differentiation and other processes, we will conduct in-depth research on the effects of external stimulation, growth environmental factors and genes on cells;● It has the characteristics of real-time monitoring, online analysis, no marking required, lossless cells, and high sensitivity;● Fundamentally improve traditional chemical methods and provide simpler, direct and more efficient means and methods for cell molecular biology research.
Application direction
●Tumor Research: Cell growth online detectors can be used for tumor research and drug development.The growth and proliferation of tumor cells differ greatly from that of normal cells, such as electrical impedance.The electrical impedance changes of tumor cells can reflect the nature, growth rate and spread of tumors, and help the diagnosis, treatment and development of tumor drugs;●Immunological research: Cell growth online detectors can also be used in immunologic research.Such as immune cell killing detection.Traditional immune killing detection is time-consuming and labor-intensive, not only requires multiple experimental testing, but also requires multiple time nodes to detect.In contrast, this instrument can monitor the killing effect of immune cells in real time and approximately simulate the immune activity in vivo.It has the characteristics of high sensitivity, high repeatability and high throughput.Simple operation, intuitive data, and can detect the killing effect of immune cells at all time nodes.It can be applied to the research on immune cell killing mechanisms, cell therapy drug research and development, etc.;●Virological research: Cell growth online detectors accurately detect the cytopathic effects of viruses in real time and in a real-time and accurate manner, which helps understand the mechanisms of virus entry, replication kinetics, and host responses in target cells.It can achieve label-free and persistent tracking and recording the progress of cytopathic effects during viral infection cells, making it convenient for a variety of virological detections, including but not limited to: virus titer determination, vaccine development, detection, quantification and anti-neutral antibodyDevelopment of viral drugs, etc.;●Cell signal transduction research: Cell signal transduction mainly focuses on cell perception, molecular pathways that transduce internal and external environmental information, and regulation of gene expression and metabolic physiological responses during biological ontogeny.Signal transduction often leads to biochemical changes that affect the number of cells present, cell size/morphology, and the tightness of cell interaction with the surface of the well plate. Cell growth can sensitively capture cell size, morphology and proliferation rate with cells.Changes in signal transduction events.This label-free continuous monitoring allows quantitative assessment of cellular responses over the first few minutes after treatment and within days;●Cell adhesion study: Cell growth online detectors can continuously monitor the adhesion dynamics of different types of cells to gain a deeper understanding of the complexity of transfer.Real-time quantitative assessment of cell adhesion and stretching without the use of fluorescent reagents or dyes, making it more convenient and simple;●Stem cell research: Cell growth online detector is a real-time, label-free, non-invasive detection method, which facilitates stem cell growth detection and drug development, and does not affect the state of the cell itself;●Research on cell barrier function: The real-time cell growth detector is a sensitive quantitative detection method that can replace traditional methods such as solute permeability detection and transendothelial cell resistance detection to detect barrier damage.
Configuration list
● Intelligent real-time cell analyzer host;
● Testing plates (2 sets of standard, one set contains 6 16-well plates);
● Intelligent real-time cell analyzer software;
● Intelligent real-time cell analyzer mobile APP (optional);
● Audit chasing software (optional).
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factory: Shanghai Liuke Broadbean Medical Technology Co., Ltd.
