Main functions

This system is the ethylene monitoring system with the highest detection limit and sensitivity in the world. It is mainly used for ethylene gas monitoring related to plant research, such as seed germination, plant growth and development, flowering physiology, plant organ aging, gene expression, plant pathogen interaction, plantHormone interactions, preservation of fruits and vegetables after receiving, research on stress resistance of plants (drought, high temperature, heavy metals), etc.

Among them, the ethylene gas detector ETD-300 adopts advanced laser technology (photoacoustic principles), that is, the sample ethylene releases heat after absorbing the laser in the photoacoustic cavity to generate pressure inside the photoacoustic cavity, and forms energy detected by the micro microphone as the laser frequency increases and decreases.The pressure difference, and the higher the ethylene concentration, the greater the pressure difference, so that the ethylene gas can be measured in real time quickly according to the difference in sound wave intensity (C)₂H₄) Absolute concentration; valve control box VC-6 is fully automated and computer-controlled. One can enable a single gas detector to achieve automatic switching measurement of 6 samples, and a single ethylene gas detector can be connected to one or more valve control boxes; hydrocarbonsDecomposition CAT-1 uses platinum particles to catalyze the oxidation and decomposition of hydrocarbons into water vapor and CO.₂, provides the system with sample air without hydrocarbon interference.

Measurement parameters

Measurement parameters: ethylene concentration (ppbv), gas flow rate (l/h), background value, analog input (V)

Calculation parameters: Ethylene yield (nl/h)

Ethylene monitoring data graphs for continuous flow assay (left) and accumulation assay (right)

Application areas

Used in the monitoring fields of environment, medicine, agriculture, industry, ecology, biology, etc.Ultrasensitive ethylene measurements are especially suitable for plant physiological and developmental studies.

Main technical parameters

Purchase Guide:

The 6-channel monitoring system is composed as follows:

Ethylene gas detector ETD-300+Valve control box VC-6+Hydrocarbon decomposition machine CAT-1

Note: All 3 instruments in the system can be used separately

Single-channel system can be selected at the discretion of:Ethylene gas detector ETD-300+ hydrocarbon decompressor CAT-1.

Origin: Sensor Sense, Netherlands

Application examples

1.1 Examples of application of ethylene assay in high temperature stress research

Introduction to the experimental content: The release of ethylene under high temperature stress was studied using Arabidopsis wild-type Col-0, mutant NahG and opr3 plants grown for 3 weeks.Among them, under the high temperature stress of wild-type Col-0 (38℃), the conductivity (electrolyte permeability), salicylic acid and jasmonic acid content and ethylene release increased; under the high temperature stress of mutants NahG and opr3 (38℃),Jasmonic acid and ethylene release also increased, but both were lower than wild-type Col-0, while the conductivity of the recovery stage after high temperature stress (22°C in water) was significantly higher than that of Col-0.The research results show that ethylene is rapidly produced under high temperature stress, and its production is regulated by jasmonic acid and salicylic acid.Overall, jasmonic acid and salicylic acid coordinate to regulate plant tolerance to high temperature stress, while ethylene mainly accelerates cell death; mutants NahG and opr3 are less heat-tolerant than wild-type Col-0 and have more cell death.

Figure 1 Salicylic acid (a), conductivity (b, c) and ethylene release (d, e) of Arabidopsis plants under high temperature treatment

WT: Arabidopsis wild type; mutant strain opr3; mutant strain NahG and medium agar

Clarke, S.M., ETA., JA is MO nates act with Sally CYLI c acid to confer basal thermo tolerance in Arab ID op sis Thalia. New pH should be mentioned in log i, 2009. 182(1):. 175-187.

1.2 Examples of application of ethylene assay in nutritional deficiency (Mg) stress research

Introduction to the experimental content: Using hydroponic Arabidopsis Col-0 plants grown for 5 weeks as material, the release of ethylene under magnesium deficiency stress was studied.After magnesium deficiency treatment, the expression levels of ethylene biosynthetase genes (e.g., At5g43450, At1g06620, and At2g25450) increased significantly, the ethylene release of samples was more than twice that of the control group, and the proportion of oxidative states of ascorbic acid ASC and glutathione GSH in the leaves increased.The research results show that there are some unique signaling pathways in plant response to magnesium deficiency stress and are related to plant hormones, while ethylene plays a key role in responding to magnesium deficiency; magnesium deficiency also simultaneously enhances plant antioxidant enzyme activity.

Table 1 Physiological parameters of newly mature leaves and roots of Arabidopsis thaliana on day 8

DHA: ASC, oxidized dehydroascorbic acid: ascorbic acid; GSSG: GSH, oxidized glutathione: glutathione; Ctrl, plants with sufficient magnesium; -Mg, plants with insufficient magnesium

Hermans, C., et al., Systems analysis of the responses to long-term magnesium deficiency and restoration in Arabidopsis thaliana. New Phytologist, 2010. 187(1): p. 132-144.

1.3 Examples of application of ethylene assay in bacterial infection research

Introduction to the experimental content: Using the mature tomato fruits with the variety Money Maker and Daniela as materials, the ethylene release of the tomato gray mold strain VTF1 was studied.The grey mold bacteria can produce ethylene in vitro, and its ethylene release is more related to mycelial growth than to be related to conidia germination, and the greater the conidia concentration, the more ethylene is released by the fungi.The ethylene release pattern of the two tomatoes infected with grey mold is similar to that of grey mold bacteria; but the release amount is 100 times higher.Combined with the cytology parameters of infected tomatoes, the results show that the ethylene release of the tomato-fungal system is not caused by the tomato grey mold bacteria, although it is very synchronized with the fungal growth rate inside it.

Figure 2 Ethylene yield of fungi (160 μl suspension)

● 1.5*108 Conidiae ml^-1▲ 2*107 Conidiae of Grey Mold ml^-1■ 2*105 Grey mildew bacteria conidia ml^-1

Figure 3 Ethylene release of two tomatoes simulated infection and different concentrations of tomato grey mildew infection

A. Tomato variety Money Maker; B. Tomato variety Daniela;

○ Simulated tomato grey mold infection ● 1.5*108 Grey mold bacteria conidia ml^-1▲ 2*107 Conidiae of Grey Mold ml^-1■ 2*105 Grey mildew bacteria conidia ml^-1

Cris espionage, S.M., ETA., ethylene production by BO try prompts CIN and EA in vitro and in tomatoes. applied and environmental microbiology, 2002. 68 (11): Fear. 5342-5350.

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