1. Product Principle

Digital PCR is an absolute quantitative detection method for nucleic acid molecules. The core principle is to achieve absolute quantitative detection technology that does not rely on standard curves through limited dilution, PCR and Poisson distribution.

Compared with qPCR technology, this system has the advantages of absolute quantification, high sensitivity and anti-inhibitor interference. In absolute quantification of pathogens, CNV copy number mutation, rare mutation detection, research on relative expression of microgenes, transgene detection, and verification of second-generation sequencing results in absolute quantification of pathogens, CNV copy number variation, rare mutation detection, and research on relative expression of microgenes, transgene detection, and verification of second-generation sequencing results in absolute quantification of pathogens,, gene editing, liquid biopsy, genetic disease detection, pathogenic microbial detection, environmental monitoring and other fields have obtained a series of research results and application transformations.

2. Product Features

1. Core Technology

1) VibroJect vibration injection technology is the world's first "active droplet generation" method.It has the characteristics of real-time monitoring, online analysis, no marking required, lossless cells, and high sensitivity.Under the action of a precision syringe pump, the reaction system to be tested flows out of the micro sample filling tip, and at the same time, the sample filling tip is reciprocating at a certain frequency. The reaction system to be tested will be divided intoUniform volume droplets.

2) The droplet size can be edited: the entire process of sample absorption and droplet generation is automated, and there is no dead volume to maximize sample utilization.

a) High resolution mode: 15ul~37k-45k

b) Standard mode: 20ul~23k

c) High sensitivity mode: 40ul~9k-11k

3. Product Advantages

1) Integrated design: equipped with droplet generation module, PCR amplification module, and fluorescent photography module.One instrument automatically completes all experiments, and the reaction liquid enters and results are out, which only takes 1.5-2 hours, and no external computer is required.

2) Simple operation: 8-connected tubes are loaded directly, and the machine is completed in 1 minute without manually transferring samples or chips.

3) Flexible sample loading: Two consumables specifications are available in 1-16/24, which meet different detection throughput requirements. The inspection of 96 samples can be completed in just 6 hours.

4) Multi-temperature control mode: high-performance temperature control module, which ensures temperature uniformity and can realize digital PCR temperature gradient.

5) 3-6 color fluorescence: can be equipped with up to 6 color fluorescence, and supports up to 21 sets of fluorescence tablet combinations, compatible with almost all fluorescence dyes.

4. Technical parameters

1) Dimension: 495mm×500mm×560mm

2) Testing time: about 1.5 hours

3) Weight: 60kg

4) Working environment requirements: temperature 15-40℃, humidity ≤85%

5) Working power supply: 100-240VAC, 50/60Hz

6) Anti-pollution design: built-in FFU circulation filtration, with ultraviolet disinfection function

7) Droplet generation method: vibration injection method

8) Reaction volume: 15-40ul

9) Detection throughput: 1-24 samples can be detected simultaneously

10) Number of effective droplets: 8000-45000 (adjust according to the mode)

11) Excitation light source: Long-life maintenance-free LED light source

12) Interaction mode: 17.3-inch built-in touch screen

13) Temperature control range: 4-98℃

14) Temperature control accuracy: ±0.1C

15) Detection method: dye method/probe method/one-step method

16) Data quality control: It has an independent QC software module that automatically controls each droplet, and can be manually viewed, modified and saved to avoid false positives and false negatives.