Pollen mass analyzer AmphaZ30

Provide you with fast and convenient single-cell characteristic analysis solutions without marking

In the process of plant breeding and production, the quality of pollen is related to the later seed yield and crop yield, and precise analysis of pollen characteristics plays an increasingly important role.At present, the most important method for studying pollen is flow cytometry analysis methods based on fluorescence staining or direct observation by microscopy. These methods take a long time to pretreat, with low flux and experimental results greatly affected by human factors.As shown in the figure on the right, when observed under a microscope, we can observe the morphological characteristics of pollen, but we cannot obtain quantitative morphological parameters; when using the flow cytometry analysis method based on fluorescence signals, the pretreatment time is long and the experimental results are repeatableLow.

In response to these situations, Amphasys developed the Ampha Z30, a pollen mass analyzer that uses impedance flow cytometry analysis technology, opening a new chapter in pollen research.The instrument greatly shortens the sample pretreatment time, and the core microfluidic analysis chip that measures the fast analysis speed and does not require dyes, greatly saving scientific researchers' time and expenses.At present, the Netherlands Ansa Seed Company has widely used Ampha Z30 to study pollen characteristics.

Application areas

Technical Principles

The core of the analysis of the pollen mass analyzer Ampha Z30 is a microchip based on the principle of Kurt counting.Compared with traditional Kurt counters, its advantages are small size and light weight (8kg).In the figure below A, after the cells (or granules) are bidirectional electrophoresis in the injection area (20x200um), the cells (or granules) are gathered in the center of the flowing liquid and arranged in a row; then the cells (or granules) pass through the red box.The marked measurement area (channel from 10x10um to 40x40um) has two different electric fields in this area. When the size, shape, and even the cell viability status of different cells (or particles) will be different, different electrical signal differences will be generated (as followsB and C in the figure, thereby calculating the number, activity and other parameters; the monitored cells (or particles) finally pass the sorting area, where there is an electric field in the opposite direction to the injection area, and the sorting area can be sorted. The cells (or particles) can be sorted.

Product composition

Pollen mass analyzer Ampha Z30 consists of 5 parts: Ampha Z30 cell counter, chips, reagents and disposables, transport boxes and control software AMPHASOFT.

youampha Z30Cell counter

Amphasys' next generation of flow cytometers is a flexible device for plug and play.It is completely based on the electrical signal analysis and processing method, no dyes and optical accessories are required, reducing installation and maintenance time.

youMicrofluidic chip

At the heart of the Ampha Z30 cell counter is a microfluidic control chip equipped with microelectrodes that can be used hundreds of times.When cells or other particles in the solution pass through a specific electric field, the chip can analyze the characteristics of the particles by detecting the resistance flowing through the liquid.To ensure measurement sensitivity, Amphas has developed chips with different channel sizes from 15-250um, covering measurements ranging from small objects (such as bacteria) to large objects (such as pumpkin, corn and cotton seeds, etc.).

youReagents and disposable products

Electrical signals are affected by the cell itself and the surrounding solutions, including solvents, additives, pH and salts.The professional reagents provided by Amphasys can process and clean a variety of samples and liquid channels, reducing the impact of contamination on experimental results.

youShipping box

The Ampha Z30 weighs only 8Kg and has a volume of 25x27x32cm. It is equipped with a 24V rechargeable battery and a 24V car adapter cable, making it fully meet laboratory and field work.

youamp ha soft

amphasoftIt integrates functional modules such as device control, data acquisition, real-time data presentation and offline data analysis.The obtained data is stored in the standard FCS3.x format. At the same time, Amphasoft for Windows can set up work tasks and calculate parameters using different analysis frequencies. The analysis tasks can be presented to users in HTML or CSSV format.

Features

ØAnalysis parameters: cell size, cell morphology, sample concentration, cell activity, cell apoptosis, cell differentiation, abiotic particle morphology parameters;

ØTest targets: bacteria, yeast (containing spores), animal and plant cells, pollen, algae;

ØOperation mode: Supports online monitoring and offline data analysis;

ØParticle size range: 15-250um;

ØSample volume: 50-800ul;

ØMeasurement time: 30-60s;

ØMeasurement speed: 1000cell/s;

ØMeasured concentration: 10-10000cell/ul;

ØMeasured flow rate: 5-400ul/min;

ØPreparation: Collect cells to be tested and diluted with buffer without staining;

ØReagent requirements: For different cells and cleaning needs, special buffer reagents and consumables are required;

ØPower supply requirements: Support laboratory or field use, 24V rechargeable battery, 24V vehicle adapter cable;

ØVolume: 255x275x353mm;

ØWeight: 8.4kg;

ØTemperature requirements: 16℃-32℃;

ØHumidity requirements: relative humidity 10%-90%;

ØPower supply: 24V_DC± 10%, max. 3A, < 90 W.

Application example

youPollen Vitality Analysis

In seed production, the credibility of pollen viability analysis is a very important factor.Experiments show that there is a clear correlation between pollen vitality and pollination and seed yield, and external environments such as temperature, light, chemicals, etc. will affect the vitality of pollen.

youPollen ploidy analysis

The ploidy and vitality of pollen were analyzed.

youAnalysis of pollen differentiation stage

Multi-frequency analysis of cell membranes within radio frequency can be used for pollen maturation stage studies.

youAnalysis of raw milk quality

In the industrialization of milk production, the quality of raw milk is very critical.When milk is affected by the mother or processing link, the quality of milk will be irreversibly affected.The Ampha Z30 cell counter can directly analyze high somatic cells in milk to identify the quality of milk.

youOnline monitoring and analysis of cell activity and quantity in bioreactors

At present, there is no method that can directly monitor the growth of cells in bioreactors on-line.The Ampha Z30 Cell Counter can help you monitor bacterial growth in fermentors in real time online.

youAnalysis of other single cells

Ampha Z30 can help users conduct simple or statistical analysis of human cells, animal and plant cells (including pollen), yeast cells (including spores), bacteria, fungi and other cells.Currently, it has been widely used in the study of bacteria, yeast, blood and cancer cells.

Company Honors

Come“The 100 most innovative and developing companies in Switzerland”

Come“W.A.de Virgil Entrepreneur Award 2013”