A fully automated PCR system establishment, amplification and analysis system! | |||||||||
IntelliQube is a fully automated and high-throughput instrument that seamlessly integrates processes such as liquid processing, thermal cycling, detection and data analysis.IntelliQube supports quantitative real-time PCR, endpoint PCR and isothermal chemistry to fully enjoy the innovative Array Tape®Consumables bring a convenient experience of inline automation. | |||||||||
Integration | |||||||||
IntelliQube integrates a variety of instruments to use during PCR to simplify your workflow, realize unmanned operation, simplified loading and operation, save laboratory space, and make researchers pay more attention to the research itself rather than the instrument.management. | |||||||||
Efficiency | |||||||||
IntelliQube and Array Tape consumables provide scalable, high-throughput solutions for your lab.Compared with the traditional 384-well qPCR instrument, the new 768-well specification doubles the PCR flux.IntelliQube and Hydrocycler for endpoint genotype detectionTMThe combination can process up to 50 microplates or 19,000 reactions per day! | |||||||||
Economical | |||||||||
Minimize reaction system (1.6uL), you can save up to 90% of your cost. | |||||||||
flexibility | ![]() | ||||||||
IntelliQube is compatible with a variety of chemical and fluorescent probes.Integration of liquid treatment improves operational flexibility.Waste is minimized compared to chip or microfluidic methods.A range of qPCR and endpoint PCR applications are compatible with this system, including but not limited to: ComeGene expression analysis ComeGenotype analysis and copy number analysis (CNVs) ComeMicrobial detection and quantitative analysis ComemiRNA analysis ComeConjugation and genetically modified organisms (GMO) detection | |||||||||
high performance | |||||||||
IntelliQube has industry-leading liquid handling, thermal cycling and optical systems that deliver the accurate PCR results you expect, with flexibility, efficiency and integration that fully meet the needs of your laboratory. | |||||||||
Liquid treatment system | ![]() | ||||||||
IntelliQube's top-notch multi-function pipetting system for quick liquid handling with 384-well or 768-well Array Tape.The operation of transferring samples to Array Tape is through interchangeable CyBi®The FeliX 96 or 384 channel pipette head can transfer reagents to the reaction system.The error of the IntelliQube liquid treatment system is less than 5%, ensuring the repeatability and data quality of the experiment. | |||||||||
Pipet rinse | |||||||||
The IntelliQube pipette flushing system can effectively reduce the consumption of pipette tips, reduce the cost of corresponding consumables, and reduce the risk of cross-contamination.This system uses fluid agitation from the pipette head to achieve the purpose of completely cleaning the inside and outside of the suction head. At the same time, its touch-closing function can offset surface tension and remove residual liquid.Each cleaning combined with cleaning additives such as bleach ensures the integrity of the detection and reduces the risk of cross-contamination. | |||||||||
Liquid treatment specifications | |||||||||
Thermal cycle | ![]() | ||||||||
IntelliQube supports inline amplification through Peltier thermal cycle blocks designed specifically for Array Tape.Thanks to the unique block design and surface geometry, no block replacement is required, and 384-hole array tape reels and 768-hole array tape reels are supported.This system has excellent heating rate, uniformity and accuracy of reaction temperature, and can be widely compatible with a variety of amplification methods, including rapid PCR and isothermal chemistry. | |||||||||
HydrocyclerTM | |||||||||
Hydrocycler combines throughput, high efficiency and stable design characteristics, and can inline amplify endpoint PCR reactants on array reels, which is another high-throughput solution to be optional.The system has 3 precisely temperature controlled water baths, optimized to fast thermal cycling for 50 arrays per run.The endpoint fluorescence signal can be read by IntelliQube after the PCR reaction, which is 5 times higher in endpoint PCR flux compared to inline thermal cycles. | |||||||||
Test | |||||||||
IntelliQube has advanced optical systems for inline fluorescence detection on ArrayTape.The filtered LEDs optimize various commonly used fluorophores with excitation ranges of 480-620 nm.The high-resolution CCD camera supports dye fluorescence intensity in 5 detection channels in 510-705nm. | |||||||||
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Testing specifications ![]() | |||||||||
Software and data analysis | |||||||||
Intellics is an innovative software product that provides IntelliQube with central data management, instrument monitoring, operation optimization, protocol generation and simplified data analysis.Intellics is preloaded on each instrument, and users can access it through a unified web-based interface that allows simple multi-user access.Intellics has two distinctive applications: Array Builder™ allows users to quickly generate liquid processing and thermal cycling protocols; IntelliScore can be used for post-PCR data analysis, supporting various PCR experiment types, including absolute quantification (standard curve), relative quantification(Relative standard curve and ΔΔCq), genotype analysis, etc.The touch screen interface is convenient for instrument control and supports real-time data monitoring. | |||||||||
Instrument Control and Software Specifications ![]() | |||||||||
Figure 1: gDNA amplification curves for 5,000 and 10,000 copies at 2x resolution ![]() | |||||||||
Figure 2: A significant Cq value change curve obtained for the entire array analysis of 10,000 copies of gDNA. ![]() | |||||||||
picture3:10plasmid with a double dilutionDNAWide dynamic range of amplification curve | |||||||||
Other performance specifications | |||||||||
Origin:UK LGC | |||||||||