Time-resolved quantitative PCR system——Archimed R6
Date:2024-12-18 16:45:54

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Technical Features:

New patented integrated optical components based on Fresnel lenses, high-efficiency silicon photomultiplier tubes and maintenance-free LEDs

1. Higher sensitivity

2. Higher repeatability and reliability

3. Resolution as low as 1.33 times the copy number difference to ensure accurate quantities

4. No system correction required

Innovative optical signal scanning method and time-resolved signal separation technology

1. Unique optical detection channel position arrangement and scanning method effectively reduce inter-hole and multi-color cross-talk

2. Based on the acquisition of fluorescent signals of the same sample and different wavelengths at different times, high-speed stepper motors and high-sensitive special detectors, multiple

Quick color board scanning

3. Each reaction well has an optimal optical path without reference dye homogeneity calibration

6-channel fluorescence detection

1. No signal interference between channels

2. Detect more than 5 target genes at the same time by the same sample to increase the amount of sample detection information

3. Cover all common fluorescence bands

Unique temperature compensation technology

1. Provide superior temperature uniformity between pores (±0.2℃) and accuracy (±0.2℃) to ensure reliable repetition of experimental data.

2. Maximum cooling rate: 6℃/second, shorten the running time and improve the experimental efficiency

Very low operating noise

1. More comfortable customer experience

2. In the cooling stage and the temperature maintenance stage, the noise of the instrument is lower than 60 decibels, and the noise of most similar products is higher than 65 decibels in the same stage.

Full Chinese interface operation software designed for Chinese users' usage habits, simple and easy to use

Cover all common data analysis methods, including absolute quantification, relative quantification, high-resolution melting curves, etc.


Configuration specifications:


1. Performance parameters:

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Technical Features:

New patented integrated optical components based on Fresnel lenses, high-efficiency silicon photomultiplier tubes and maintenance-free LEDs

1. Higher sensitivity

2. Higher repeatability and reliability

3. Resolution as low as 1.33 times the copy number difference to ensure accurate quantities

4. No system correction required

Innovative optical signal scanning method and time-resolved signal separation technology

1. Unique optical detection channel position arrangement and scanning method effectively reduce inter-hole and multi-color cross-talk

2. Based on the acquisition of fluorescent signals of the same sample and different wavelengths at different times, high-speed stepper motors and high-sensitive special detectors, multiple

Quick color board scanning

3. Each reaction well has an optimal optical path without reference dye homogeneity calibration

6-channel fluorescence detection

1. No signal interference between channels

2. Detect more than 5 target genes at the same time by the same sample to increase the amount of sample detection information

3. Cover all common fluorescence bands

Unique temperature compensation technology

1. Provide superior temperature uniformity between pores (±0.2℃) and accuracy (±0.2℃) to ensure reliable repetition of experimental data.

2. Maximum cooling rate: 6℃/second, shorten the running time and improve the experimental efficiency

Very low operating noise

The instrument noise is lower than 60 decibels in the cooling stage and the temperature maintenance stage, and the noise is higher than 65 decibels in the same stage of most similar products.

Full Chinese interface operation software designed for Chinese users' usage habits, simple and easy to use

Cover all common data analysis methods, including absolute quantification, relative quantification, high-resolution melting curves, etc.


Configuration specifications:


1. Performance parameters:


Arch IME's 384High-throughput fluorescence quantificationPCRInstrument performanceparameter

Sensitivity

Minimum 1 copy

Resolution

1.33 times the copy number difference can be distinguished in single-fold reaction

Linear dynamic range

10 orders of magnitude (1-1010copy)

Sample detection repetition

Ct value CV≤0.5% or Ct SD≤0.1

Sample Linear

day≥0.98 or r2≥0.99

Multiple analysis performance

Can detect 5 to 6 targets simultaneously

Analysis Methods

Absolute Quantity Relative Quantity Qualitative Analysis Endpoint Fluorescence Analysis Melting Curve High Resolution Melting Curve (HRM)

Genotype (SNP) Protein Thermal Stability


2. Hardware parameters:

Arch IME Dr6Time-resolved quantificationPCRSystem hardware parameters

Sample capacity

96

Reaction volume

5-100µCome

Thermal cycling technology

Peltier (6 temperature control modules)

Temperature control technology

Hollow module combined with edge temperature compensation technology

Heat cover technology

Electronic automatic thermal cover (at room temperature -115℃ adjustable)

Maximum cooling rate of module

≥6.0℃/s

Average sample cooling rate

≥3.0℃/s

Temperature range

4 - 100℃

Temperature accuracy

≤ ±0.1℃

Temperature uniformity

≤ ±0.1℃

Temperature gradient range

30 - 100℃

Number of temperature gradients

12 cases

Temperature gradient temperature range

1 - 40℃

Reaction run time

<30 minutes (according to the reaction reagent)

Excitation light source

6 monochrome high-efficiency LEDs (maintenance-free, working life >100,000 hours)

Detection device

Large size high sensitivity silicon photomultiplier tube (MPPC)

Arch IME Dr6Time-resolved quantificationPCRSystem hardware parameters

Detection mode

Time-resolved real-time scanning

Test time

Standard mode (full channel): 8.5 seconds/96-well plate

Fast Mode (Dual FAM): 4 seconds/96-well plate

Fluorescence reading position

Top Read

Number of fluorescence channels

6

Fluorescence band range

Excitation range: 455-680nm Detection range: 510-730nm

Supported dyes

F1: FAM/sy br green i  F2: Vic/hex/TE T/Joe  F3:RO X/Texas red

F4: Cy5/LIZ                   F5: Cy3/TAMRA or Alexa Fluor 680/Cy5.5

F6: FAM/SYBR Green I or FRET

Data communication interface

USB

Computer configuration

The mainstream brand desktop computer or large-size touch screen computer for Windows operating systems

Equipment volume

52×35×37cm (Length×Width×Height)

Equipment weight

25 Viewed

Operating temperature

5-32℃

Working humidity

≤85%

Operating voltage

100-240 vac, 50-60 Hz


3. Software parameters:

Arch IME Dr6Time-resolved quantificationPCRSystem software parameters

Supporting software

Arch IME's analyzer

Program run

Program Setup Wizard or customer designs the program yourself

MIQE Guide

conform to

Data Export

It can export CSV, Excel, txt and other formats, and user reports include experimental attributes, operation settings,

Data results, original data and other information; graphic and tabular data results can be printed directly or

Save it in PDF format; customize the experimental report format; pre-store multiple experimental report modules


Application areas:

Relative quantitative analysis

1. Comparison of gene expression levels in different tissues

2. Comparison of gene expression levels between treated and untreated samples

3. Comparison of the expression levels of genes of interest under different genetic backgrounds

4. Changes in gene expression over time under special conditions

Absolute quantitative analysis

1. Determine the number of copies of genes in a certain amount of unknown samples

2. Test the concentration or quantity of virus particles per milliliter of blood in the patient

3. Detection of the content of genetically modified nucleic acid in genetically modified foods

Protein thermal stability analysis

1. Improve protein preparations (buffer, pH, salt, auxiliary materials)

2. Formation conditions conducive to crystallization

3. Develop protein formulas and storage buffers

4. Analyze the effects of mutations or modifications

Probe melting curve analysis

1. Genotyping of multiple genologic sites

2. Mutation screening for random mutations

3. Identification of subtypes of gene polymorphic regions

4. Simultaneous detection of multiple missing types

Endpoint fluorescence (anotypic identification) analysis

1. Compare whether different samples contain pathogenic microorganisms

2. Compare whether the sample contains a specific species

3. Compare whether the sample contains a specific variant or isomer

4. Biological residues in drugs or biological agents

Genotyping analysis

1. Genetic Disease Genetic

2. Identification of tumor susceptibility genes and hotspot mutations

3. Identification of pathogen gene mutations and drug resistance

4. Animal and plant evidence collection and breeding research

Nucleic acid melting curve analysis

1. Distinguish specific from nonspecific products (primer dimer)

2. Determine the Tm value of the target gene

High resolution melting curve (HRM) analysis

1. Identify unknown mutations and SNPs of genes;

2. Genotyping of known mutations and SNPs;

3. Species identification and variety identification of species such as animals, plants, microorganisms;

4. Forensic identification and paternity identification;

5. HLA matching;

6. Research on methylation

African swine fever virus detection





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