LGC High Throughput Genotyping System
DNA extraction
The Oktopure platform is a high-throughput animal and plant tissue automatic extractor based on magnetic bead method, which can perform DNA extraction from 8×96 well plates in parallel; Standardized extraction procedures can obtain high-quality DNA, which can meet the quality requirements of most molecular biology experiments for DNA, including first-generation sequencing, second-generation sequencing and genotyping, such as KASP technology; The platform is equipped with 96-channel pipettes for automated transfer; 8 automatic magnetic racks that secure the beads to the bottom of the 96-well plate during liquid transfer. |  |
Magnetic bead extraction that improves automation efficiency and flux When the starting sample size is 20-30 mg, it takes approximately 1.5 hours to complete DNA extraction from 8 96-well plates; When the starting sample size is 80-100 mg, it takes approximately 2.5 hours to complete DNA extraction from 8 96-well plates; DNA extraction flux can reach 3,500 samples per day; Suitable for leaves and seeds of plants, cells and tissues of animals, and microorganisms; Offline washing systems reduce costs Reusable after cleaning the gun head, thereby reducing the cost of consumables Matching downstream application instruments For example, genotyping platforms, first-generation and second-generation sequencing machines
Why choose Oktopure?
How Sbeadex works
Genespin™ High Throughput DNA Extractor
Genespin platform Kleargene kit based on filter membrane method, semi-automated high-throughput extraction of DNA using 96 or 384-well plates as carriers.Obtaining high-quality DNA according to the standardized extraction process can meet most experimental needs based on PCR technology.The platform is equipped with 96 or 384 channel pipettes and can also be used for automated liquid transfer.
Tablet copy
repliKator fully automatic DNA board replication workstation
Replikator was originally used to address the challenges of a large number of template replications we face in the genotyping service lab.RepliKator uses precise 96/384 channel pipette heads, gun head cleaning stations and intuitive software control interfaces (RS232 controllable), repliKator can quickly replicate 96, 384 and 1536 well plates or transfer samples from 96 to 384 well platesand 1536 microplate. |  |
Suitable for 96, 384 and 1536-hole SBS format panels
Adjust the height of the pipette head, compatible with shallow and deep-hole plates
24 1536-well PCR plates can be prepared in 5 minutes
Disposal range: 0.5–500 μL, the pipette can be reused
Separation accuracy: CV (coefficient of variation) <3% (1.0 μL)
Equipped with automatic gun head cleaning station
Compatible with Kraken. Laboratory Management and Classification Software
Intuitive software interface, simple and convenient operation process
K-pette semi-automatic DNA board replication workstation
The K-pette semi-automatic DNA plate replication workstation uses an advanced Apricot design system to accurately and efficiently perform well plate replication.It includes a 384 channel pipette that can function as a 96 or 384 pipette with a separation range of 1 μL–125 μL, CV < 5%.Through the touch screen interface, all operations can be easily completed.Use semi-automated gun head cleaning workstations to realize reuse of gun heads, further saving costs and improving efficiency. |  |
Use a flat plate adapter to easily achieve the following pipettingmodel: 96 to 96 96 to 384 96 to 1536 384 to 384 384 to 1536 Liquid aliquot
Meridian2 trace liquid dispenser
The Meridian2 trace dispenser is an automated high-throughput liquid dispensing station equipped with an 8-channel nozzle that distributes 1 μL of PCR reaction mixture into 1536 microplate in 45 seconds.
Key Advantages
Easy to operate
No disposable gun tips required to reduce costs
Powerful valve design, can complete more than 4 million liquid dispensing times
Non-contact liquid separation eliminates cross-contamination
1 μL of the reaction mixture can be distributed to 1536 in 45 seconds
In microplate
Separation accuracy: 1 μL, CV < 5%
Intuitive software interface makes liquid separation easy and fast
Flat plate seal
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Why choose Kube?
Compatible tablet height: 9–45 mm;
Tablet sealing time: 6-8 seconds;
A film roll with a diameter of 265 mm can be sealed for more than 4500 times;
Use a simple and intuitive touch screen for operation;
Built-in multiple sealing programs to choose from;
The instrument is smaller in size, saving space;
The high skirt plate can be sealed.
Fusion3™ Laser Sealer
No heat is transferred to the sample, ensuring the accuracy of the experiment; Sealable with higher density flat plates (1536 microplate); An optically transparent film may be used to increase the signal-to-noise ratio. |  |
Why choose Fusion3?
Compatible with 96, 384 and 1536 well plates;
After sealing, it can withstand high temperature reaction conditions and long-term storage;
Can extend the incubation time;
Eliminate evaporation-based edge effect compatible with optical transparent film or coloring film seals, and improve the detection effect of primers according to customer needs;
Ideal for photosensitive substances;
There is no risk of PCR product contamination, so no division is required;
PCR front-end and PCR back-end areas.
Thermal cycle
Hydrocycler2 —— Water Bath PCR Thermal Cyculator
Water Thermal Conductivity: Using water to conduct heat to PCR reaction systems has many advantages: it can simultaneously process a large number of orifice plates or array tapes (Array Tapes) to achieve high throughput; it can transfer between plates, array tapes and array tapes.The temperature difference decreases. Shorten the experimental time: Since there is no need to increase or cool down during the PCR cycle, the cycle time is 40% shorter than that of traditional methods. Accurate temperature control: Three separate water bathtubs are included, each of which can be precisely controlled.Depending on the different PCR reaction procedures, the orifice plate or array tape can be automatically switched to a water bath of different temperatures. |  |
Why choose Hydrocycler2?
Improve throughput
Ability to process more than 145,000 samples in one 8-hour working day
384-hole array belt for 96, 384 and 1536-well plates, Nexar® and IntelliQube®
Efficient expansion
Cyclic jet ensures uniform temperature in each water bath, thereby eliminating edge effects to make PCR reactor conditions highly consistent
Multi-water bath system allows precise temperature control, shortens cycle time and eliminates temperature fluctuations
No special ambient temperature required
Compact design, small instrument size, can be placed on the floor or on the laboratory bench, and the water bath cover can reduce the amount of water evaporation
Compatible with various orifice plates and array tapes
Intuitive user interface
Simple and intuitive user interface is easy to operate
Can realize barcode tracking function
Includes report generation and log file viewer
Chemical reagents and experimental protocols that support all common endpoint PCR and emulsion PCR (ePCR)
Provide uniform temperatures for the entire orifice plate or array strip, enabling synchronous reactions and obtaining accurate and consistent data
The flux level depends on the specific cycle conditions and the choice of chemical reagents.This estimated value is based on the standard KASP reaction of 1536 microplate for 1 hour and 10 minutes.
SNP typing detector
pH and A star Pherastar SNP typing detectors can be used for 96, 384 andKASP SNP typing for 1536 well plates and data analysis and management can be performed through our Kraken™ software.PHERAstar uses single excitation light and dual emission light detection to simultaneously read FAM and ROX fluorescent signals in a single acquisition, and then simultaneously read HEX and ROX fluorescent signals in the second acquisition. This technology improves efficiency by 30%.A 1536 microplate can be read in 1 minute and 20 seconds, and the fluorescence detection sensitivity reaches 0.2 fmol fluorescent molecules/well.While purchasing this instrument, we will configure the typing software KlusterCaller™, the software can enter the SNP typing resultsAutomatic analysis.PHERAstar is fully functional to meet our needs for high-throughput SNP typing detection.
| omega F This detector is suitable for KASP SNP typing detection of 96 and 384-well plates. It can detect fluorescence such as FAM, HEX and ROX. It can read a 96-well plate in 10 seconds and a 384-well plate in 66 seconds.While purchasing this instrument, we will configure the typing soft KlusterCaller™, which can automatically analyze the typing results of SNPs.
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